Time-Dependent Evaluation of Diazinon-Induced Haematological Alterations and Their Amelioration by Aloe barbadensis in Albino Rats
DOI:
https://doi.org/10.59436/ijpsr.v2i3.8.3139-342XKeywords:
Diazinon; Aloe barbadensis; haematology; Wistar rats; exposure duration; erythrocytesAbstract
The present experiment was designed to evaluate the time dependent hematological changes induced by repeated oral exposure of diazinon and protective role of Aloe barbadensis in male Wistar albino rats. The median lethal dose (LD50) of diazinon was estimated to be approximately 300 mg/kg body weight, and one-tenth of this dose (30 mg/kg) was chosen for repeated exposure. The experimental animals were divided into control, diazinon, diazinon + Aloe barbadensis and recovery groups. Hematological parameters were assessed on 7th, 14th, 21st and 28th day. Hemoglobin (Hb), total erythrocyte count (TEC), total leucocyte count (TLC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and erythrocyte sedimentation rate (ESR) were the parameters studied. Hematological indices showed progressive changes following exposure to Diazinon, characterized by decreased hemoglobin concentration, erythrocyte count, packed cell volume, MCH and MCHC and increased leucocyte count, MCV and ESR. The effects were further enhanced with increased duration of exposure indicating cumulative haematotoxic effects. Administration of Aloe barbadensis normalized several diazinon-induced abnormalities and the recovery group showed generally better improvement toward control values. Although these mechanisms were not directly investigated, the observed protective responses may be attributed to the antioxidant and cytoprotective constituents of Aloe barbadensis. The results indicated that repeated exposure of diazinon disturbs the hematological homeostasis and Aloe barbadensis might reduce the associated alterations. Further experimental validation is needed to identify the underlying protective mechanisms and to establish reproducibility in independent animal studies.
References
Abdollahi, M., Ranjbar, A., Shadnia, S., Nikfar, S., & Rezaie, A. (2004). Pesticides and oxidative stress: A review. Medical Science Monitor, 10(6), RA141–RA147.
Costa, L. G. (2018). Organophosphorus compounds at 80: Some old and new issues. Toxicological Sciences, 162(1), 24–35. https://doi.org/10.1093/toxsci/kfx266
Finney, D. J. (1971). Probit analysis (3rd ed.). Cambridge University Press.
Halliwell, B., & Gutteridge, J. M. C. (2015). Free radicals in biology and medicine (5th ed.). Oxford University Press.
Hamman, J. H. (2008). Composition and applications of Aloe vera leaf gel. Molecules, 13(8), 1599–1616. https://doi.org/10.3390/molecules13081599
Jain, N. C. (1986). Schalm's veterinary hematology (4th ed.). Lea & Febiger.
Klaassen, C. D. (Ed.). (2019). Casarett & Doull's toxicology: The basic science of poisons (9th ed.). McGraw-Hill Education.
Mostafalou, S., & Abdollahi, M. (2017). Pesticides: An update of human exposure and toxicity. Archives of Toxicology, 91, 549–599. https://doi.org/10.1007/s00204-016-1849-x
National Research Council. (2011). Guide for the care and use of laboratory animals (8th ed.). National Academies Press. https://doi.org/10.17226/12910
Reynolds, T., & Dweck, A. C. (1999). Aloe vera leaf gel: A review update. Journal of Ethnopharmacology, 68(1–3), 3–37. https://doi.org/10.1016/S0378-8741(99)00085-9
Surjushe, A., Vasani, R., & Saple, D. G. (2008). Aloe vera: A short review. Indian Journal of Dermatology, 53(4), 163–166. https://doi.org/10.4103/0019-5154.44785
Tukey, J. W. (1949). Comparing individual means in the analysis of variance. Biometrics, 5(2), 99–114. https://doi.org/10.2307/3001913
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Lata Fauzdar, Dr. Sonal Singh, Dr. Anand Pratap Singh (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
